Description
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-LeptinR polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-LeptinR polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LeptinR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LeptinR can be calculated.
Background: Leptin receptor also known as LEP-R is a protein that in humans is encoded by the LEPR gene. It is a member of the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. The leptin receptor is found in many tissues in several alternatively spliced forms, raising the possibility that leptin exerts effects on many tissues including the hypothalamus. The leptin hormone regulates adipose-tissue mass through hypothalamus effects on fullness and energy use. Variations in the leptin receptor have been associated with obesity and with increased susceptibility to Entamoeba histolytica infections